Fucosylglycolipids in cells transformed by a temperature-sensitive mutant of murine sarcoma virus

WE have reported that fucosylglycolipid metabolism is markedly altered in cells transformed by oncornavirus 1 . Transformed cells exhibit a sharp decrease in radioactive fucose incorporation into a chromatographically less mobile fucosylglycolipid, with a corresponding increase in radioactivity in c...

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Bibliographic Details
Published in:Nature (London) 1974-04, Vol.248 (5450), p.682-684
Main Authors: STEINER, SHELDON M, MELNICK, JOSEPH L, KIT, SAUL, SOMERS, KENNETH D
Format: Article
Language:English
Subjects:
Animals
Autoradiography
Carbon Radioisotopes
Cell Line
Cell Transformation, Neoplastic
Cells, Cultured
Chromatography, Thin Layer
Fucose - metabolism
Gammaretrovirus - metabolism
Glycolipids - analysis
Glycolipids - biosynthesis
Glycolipids - metabolism
Humanities and Social Sciences
Kidney
letter
Mice
multidisciplinary
Mutation
Phenotype
Rats
Science
Science (multidisciplinary)
Temperature
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Summary:WE have reported that fucosylglycolipid metabolism is markedly altered in cells transformed by oncornavirus 1 . Transformed cells exhibit a sharp decrease in radioactive fucose incorporation into a chromatographically less mobile fucosylglycolipid, with a corresponding increase in radioactivity in chromatographically more mobile fucosylglycolipids. We describe here further studies of fucosylglycolipid metabolism using normal rat kidney (NRK) cells transformed by a cold-sensitive mutant of murine sarcoma virus (MSV) 2 . The cell line, designated NRK (MSV-1b), is cold sensitive for maintenance of the transformed phenotype. The cells express the transformed phenotype at the permissive temperature (39° C) but appear phenotypically normal at the non-permissive temperature (33° C). Cells transformed by this mutant are non-producer cells, but contain the MSV genome in a rescuable form. Using these cold-sensitive transformants, we were able to demonstrate, in a temporal fashion, a relationship between altered fucosylglycolipid metabolism and the development of the transformed phenotype.
ISSN:0028-0836
1476-4687
DOI:10.1038/248682a0