Infectious bursal disease virus: growth and characterization in cell cultures

The infectious bursal disease virus (IBDV) was adapted to chicken embryo bursal (CEB) and kidney (CEK) cells. The virus did not replicate in the kidney cells until 4 serial passages of the virus were made in bursal cells. The virus produced plaques in CEK cells in 3-5 days. The replication of the vi...

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Bibliographic Details
Published in:Avian diseases 1974-04, Vol.18 (2), p.243-250
Main Authors: Lukert, P.D, Davis, R.B
Format: Article
Language:English
Subjects:
Animals
Bursa of Fabricius
Cell culture techniques
Cell growth
Cells, Cultured
Chick Embryo
Chickens
Chloroform - pharmacology
Culture Media
Cultured cells
Embryos
Fluorescent Antibody Technique
Hot Temperature
Idoxuridine - pharmacology
Infectious bursal disease virus
Infectious diseases
Kidney cells
Microscopy, Electron
Poultry Diseases - microbiology
Reoviridae - drug effects
Reoviridae - growth & development
Reoviridae - immunology
Reoviridae - isolation & purification
Reoviridae Infections - microbiology
Reoviridae Infections - veterinary
Serial passage
Viral Plaque Assay
Viruses
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Summary:The infectious bursal disease virus (IBDV) was adapted to chicken embryo bursal (CEB) and kidney (CEK) cells. The virus did not replicate in the kidney cells until 4 serial passages of the virus were made in bursal cells. The virus produced plaques in CEK cells in 3-5 days. The replication of the virus was unaltered by 5-iodo-2′-deoxyuridine, indicating that it is a ribovirus. The infectivity of the virus was not destroyed with chloroform and was stable at 56 C for 90 min. The virus attached to the CEK cell monolayer maximally in 75 min and had an eclipse period of 9-10 hours before virus maturation and release. Immunofluorescent studies of infected cells showed that the first antigen was detected 6 hours postinfection and all fluorescing antigen was confined to the cytoplasm. All of these characteristics lend support to the classification of IBDV in the diplornavirus group of riboviruses.
ISSN:0005-2086
1938-4351
DOI:10.2307/1589133