Replication and Transcription of Eukaryotic DNA in Escherichia coli

Fragments of amplified Xenopus laevis DNA, coding for 18S and 28S ribosomal RNA and generated by EcoRI restriction endonuclease, have been linked in vitro to the bacterial plasmid pSC101; and the recombinant molecular species have been introduced into E. coli by transformation. These recombinant pla...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1974-05, Vol.71 (5), p.1743-1747
Main Authors: Morrow, John F., Cohen, Stanley N., Annie C. Y. Chang, Boyer, Herbert W., Goodman, Howard M., Helling, Robert B.
Format: Article
Language:English
Subjects:
Animals
Biochemistry
Buoyancy
DNA
DNA - analysis
DNA Replication
Drug Resistance, Microbial
Electrophoresis
Escherichia coli - metabolism
Extrachromosomal Inheritance
Gels
Microscopy, Electron
Molecular Weight
Molecules
Nucleic Acid Hybridization
Phosphorus Radioisotopes
Plasmids
Recombination, Genetic
Ribosomal DNA
Ribosomal RNA
RNA
RNA, Bacterial
Tetracycline
Transcription, Genetic
Transformation, Genetic
Tritium
Ultracentrifugation
Xenopus
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Summary:Fragments of amplified Xenopus laevis DNA, coding for 18S and 28S ribosomal RNA and generated by EcoRI restriction endonuclease, have been linked in vitro to the bacterial plasmid pSC101; and the recombinant molecular species have been introduced into E. coli by transformation. These recombinant plasmids, containing both eukaryotic and prokaryotic DNA, replicate stably in E. coli. RNA isolated from E. coli minicells harboring the plasmids hybridizes to amplified X. laevis rDNA.
ISSN:0027-8424
DOI:10.1073/pnas.71.5.1743