Glucose- cyclo -Acetoacetate as a Precursor of Ascorbic Acid in Germinating Green Gram

THAT glucose- cyclo -acetoacetate acts as a precursor of ascorbic acid in germinating green gram or mung beans ( Phaseolus radiatus ) has been shown by Nath and his associates 1–3 and confirmed by the use of labelled glucose- cyclo -acetoacetate by Thangamani and Sarma 4 . Neither the titrometric in...

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Bibliographic Details
Published in:Nature (London) 1959-05, Vol.183 (4670), p.1258-1259
Main Authors: NATH, M. C, BELKHODE, M. L
Format: Article
Language:English
Subjects:
Acetoacetates
Ascorbic Acid - metabolism
Carbohydrate Metabolism
Fabaceae
Germination
Glucose - analogs & derivatives
Humanities and Social Sciences
letter
multidisciplinary
Old Medline
Science
Science (multidisciplinary)
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Summary:THAT glucose- cyclo -acetoacetate acts as a precursor of ascorbic acid in germinating green gram or mung beans ( Phaseolus radiatus ) has been shown by Nath and his associates 1–3 and confirmed by the use of labelled glucose- cyclo -acetoacetate by Thangamani and Sarma 4 . Neither the titrometric indophenol dye method of Bessey and King 5 nor the colorimetric method of Roe and Kuether 6 is specific for L -ascorbic acid. We have therefore used paper chromatography to identify the additional reducing substance formed from glucose- cyclo -acetoacetate during the process of germination which decolorizes the indophenol dye, and find it to be L -ascorbic acid.
ISSN:0028-0836
1476-4687
DOI:10.1038/1831258a0