Cytochemical and Morphologic Identification of Macrophages and Eosinophils in Tissue Cultures of Normal Human Bone Marrow

When normal human bone marrow cells are cultured in soft agar, in the presence of human spleen-conditioned medium, two types of colonies could be recognized by means of the inverted microscope: large colonies consisting of macrophagesmonocytes, and small colonies consisting of eosinophils. In the tw...

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Bibliographic Details
Published in:Blood 1974-08, Vol.44 (2), p.221-233
Main Authors: Shoham, D., David, E. Ben, Rozenszajn, L.A.
Format: Article
Language:English
Subjects:
Acid Phosphatase - metabolism
Alkaline Phosphatase - metabolism
Arginine
Bone Marrow
Bone Marrow Cells
Cells, Cultured
Eosinophils
Esterases - metabolism
Histocytochemistry
Humans
Macrophages - enzymology
Microscopy, Electron
Microscopy, Phase-Contrast
Naphthaleneacetic Acids
Peroxidases
Spleen - cytology
Staining and Labeling
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Summary:When normal human bone marrow cells are cultured in soft agar, in the presence of human spleen-conditioned medium, two types of colonies could be recognized by means of the inverted microscope: large colonies consisting of macrophagesmonocytes, and small colonies consisting of eosinophils. In the two types of colonies, cells developing from blasts to mature cells could be recognized by their morphologic and cytochemical properties. The identification of macrophage-monocytes and of eosinophils was based on light, phase-contrast, and electron microscopy, the strong positive reaction of α-naphthyl acetate esterase in the macrophagemonocyte, and the positive stain with Luxol Fast Blue, eosin, and peroxidase of the eosinophils.
ISSN:0006-4971
1528-0020
DOI:10.1182/blood.V44.2.221.221