GLC Determination of Chlorphenesin Carbamate in Serum

A method for the determination of chlorphenesin carbamate in human serum was developed to measure the concentrations of intact drug in circulation after therapeutic doses (⩽400 mg). The method, based on a chloroform extraction of the buffered (pH 7.6) specimen and subsequent GLC analysis of the trim...

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Bibliographic Details
Published in:Journal of pharmaceutical sciences 1974-07, Vol.63 (7), p.1094-1097
Main Authors: Kaiser, D.G., Shaw, S.R.
Format: Article
Language:English
Subjects:
Adult
Carbamates - blood
Chlorphenesin - blood
chlorphenesin carbamate in human serum
Chlorphenesin carbamate-GLC analysis in human serum
Chromatography, Gas
Drug Stability
GLC-analysis
GLC-analysis, chlorphenesin carbamate in human serum
Half-Life
Humans
Indicators and Reagents
Male
Mass Spectrometry
Methods
Reaction Time - drug effects
Time Factors
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Summary:A method for the determination of chlorphenesin carbamate in human serum was developed to measure the concentrations of intact drug in circulation after therapeutic doses (⩽400 mg). The method, based on a chloroform extraction of the buffered (pH 7.6) specimen and subsequent GLC analysis of the trimethylsilyl derivaties of the extract residue, is sensitive to 0.5 μg chlorphenesin carbamate/ml serum. Statistical analyses indicate an average recovery (±SD) of 98.3 ± 7.8%. Mass spectrometric analysis, in conjunction with GLC, confirmed the specificity of the method for intact drug. The procedure was successfully applied to drug absorption studies in humans.
ISSN:0022-3549
1520-6017
DOI:10.1002/jps.2600630713