“Feeding the Baby”-Designing the Culture Milieu to Enhance Cell Stability

Attention was drawn to the responsiveness of cells, in vivo and in vitro, to small changes in their environment. Some of the more important, and the more often neglected, influences of the milieu on the genotypic and phenotypic stability and instability of cells in vitro were reviewed. Among the fac...

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Bibliographic Details
Published in:JNCI : Journal of the National Cancer Institute 1974-11, Vol.53 (5), p.1443-1448
Main Author: Waymouth, C
Format: Article
Language:English
Subjects:
Adrenal Cortex Hormones
Amino Acids
Blood
Calcium
Cell Transformation, Neoplastic
Cells, Cultured
Culture Media
Culture Techniques
Genotype
Growth Substances
Hydrogen-Ion Concentration
Insulin
Membrane Potentials
Oxygen
Partial Pressure
Phenotype
Potassium
Purines
Pyrimidines
Trace Elements
Vitamins
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Summary:Attention was drawn to the responsiveness of cells, in vivo and in vitro, to small changes in their environment. Some of the more important, and the more often neglected, influences of the milieu on the genotypic and phenotypic stability and instability of cells in vitro were reviewed. Among the factors affecting one or more forms of cell stability were microorganisms (sometimes unrecognized), serum proteins, enzymes, cations, ionic balance and pH, O2 tension, hormones, and macromolecular growth .factors. It was suggestedthat high priority should be given to the design of defined milieus for enhancing the genotypic and phenotypic stability of specific cell types, and to the elimination of the hazards, variabilities, and uncertainties associated with the use of serum in culture media.
ISSN:0027-8874
1460-2105
DOI:10.1093/jnci/53.5.1443