Structural Damage of Bacteriophage T1 by Surface Inactivation

Laboratory of Microbiology, State University, Catharijnesingel 59, Utrecht, The Netherlands, and National Institute of Public Health, P.O. Box 1, Bilthoven, The Netherlands The structure of bacteriophage T 1 was examined after inactivation by rotating the virus suspension in a rotating bulb or by bu...

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Bibliographic Details
Published in:Journal of general virology 1974-10, Vol.25 (1), p.75-81
Main Authors: Trouwborst, T, Kuyper, Sjoukje, Teppema, J. S
Format: Article
Language:English
Subjects:
Air
Carbon Radioisotopes
Centrifugation, Density Gradient
Coliphages - pathogenicity
Coliphages - ultrastructure
DNA Viruses
DNA, Viral - biosynthesis
Escherichia coli
Hot Temperature
Microscopy, Electron
Rotation
Thymidine
Tritium
Viral Proteins
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Summary:Laboratory of Microbiology, State University, Catharijnesingel 59, Utrecht, The Netherlands, and National Institute of Public Health, P.O. Box 1, Bilthoven, The Netherlands The structure of bacteriophage T 1 was examined after inactivation by rotating the virus suspension in a rotating bulb or by bubbling gas through the suspension. After inactivation the [ 3 H]-label of the nucleic acid was located in CsCl and sucrose gradients at the site of free DNA. The small fraction of phage which is resistant to surface inactivation had the same sedimentation coefficient and temperature sensitivity as the control phage, indicating that resistance is not due to agglomerate formation. No morphological differences between phage particles before and after inactivation could be demonstrated by electron microscopy. * Present address: Du Pont de Nemours, Dordrecht, the Netherlands. Received 6 March 1974; accepted 7 June 1974.
ISSN:0022-1317
1465-2099
DOI:10.1099/0022-1317-25-1-75