Sequence studies of nonradioactive Mycoplasma tRNA Phe with the aid of polynucleotide phosphorylase and polynucleotide kinase

The known methods of enzymatic phosphorylation with [(32)P]phosphate of the 3'- or 5'-hydroxyl group of an oligonucleotide have been applied to oligonucleotides derived from Mycoplasma tRNA(Phe). The fingerprints obtained by both methods are very similar to each other and to that of unifor...

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Bibliographic Details
Published in:Nucleic acids research 1974-12, Vol.1 (12), p.1733-1738
Main Authors: Szeto, K S, Soll, D
Format: Article
Language:English
Subjects:
Base Sequence
Micrococcus - enzymology
Mycoplasma - analysis
Phenylalanine
Phosphorus Radioisotopes
Phosphotransferases
Polynucleotides
Polyribonucleotide Nucleotidyltransferase
Ribonucleotides - analysis
RNA, Bacterial - analysis
RNA, Transfer - analysis
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Summary:The known methods of enzymatic phosphorylation with [(32)P]phosphate of the 3'- or 5'-hydroxyl group of an oligonucleotide have been applied to oligonucleotides derived from Mycoplasma tRNA(Phe). The fingerprints obtained by both methods are very similar to each other and to that of uniformly labelled tRNA. The sequence of some oligonucleotides was determined by partial digestion of the 3'-phosphorylated fragment with spleen phosphodiesterase and of the corresponding 5'-phosphorylated fragment with venom phosphodiesterase.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/1.12.1733