Studies in Leukemia. XII. Interference and Reactivation Experiments with Cell-Free Tissue Extracts of Leukemic Swiss Mice

• Published in: Blood 1960-01, Vol.15 (1), p.95-102
• Main Authors: Sinkovics, Joseph G., Spurrier, Wilma A., Schwartz, Steven O.
• Format: Article
• Language: English
• Subjects: Animals; Diploidy; Leukemia; Leukemia, Experimental; Old Medline; Tissue Extracts
• ISSN: 0006-4971; 1528-0020
• DOI: 10.1182/blood.V15.1.95.95

1. The virulence of the Swiss mouse leukemia agent can be enhanced by rapid brain to brain passages. 2. The existence of an occult agent in the tumor tissue is demonstrable by interference and reactivation experiments. 3. Tumor extracts inhibit the leukemogenic activity of brain homogenates of leukemic mice. 4. Mixtures of tumor extracts and heat-inactivated brain extracts, neither of which is capable of inducing leukemia alone, have leukemia-inducing activity. 5. A heat-labile inhibitor is probably responsible for the masking of the virus in tumor extracts. 1. Le virulentia tia del agente de leucemia in muses switze pote esser promovite per rapide passages ab cerebro a cerebro. 2. Le existentia de un occulte agente in le tissus tumoral es demonstrabile per experimentos de interferentia e reactivation. 3. Extractos de tumor inhibi le activitate leucemiogene de homogenatos cerebral de muses leucemie. 4. Mixturas de extractos tumora con thermo-inactivate extractos cerebral possede un activitate de induction de leucemia ben que ni le extractos cerebral ni le extractos tumoral es capace per se a effectuar un tal induction. 5. Un thermo-labile inhibitor es probabilemente responsabile pro le mascation del virus in extractos tumoral.