Evaluation of Media Used for Cultures of Neisseria gonorrhoeae and Comparison of Commercial and Laboratory Prepared Supplements

A system for the preliminary evaluation of media to detect Neisseria gonorrhoeae is described. A dissecting microscope fitted with an eyepiece reticulum was used to measure colony diameters at 18 and 22 hours, and clinical trials in a health department venereal disease clinic were used to determine...

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Bibliographic Details
Published in:Journal of the American Venereal Disease Association 1974-09, Vol.1 (1), p.14-19
Main Authors: CHANDLER, ROBERT W., RENDTORFF, ROBERT C., CURRAN, JAMES W., KELLOGG, DOUGLAS S.
Format: Article
Language:English
Subjects:
Cervix Uteri - microbiology
Culture Media
Evaluation Studies as Topic
Female
Gonorrhea - microbiology
Humans
Neisseria gonorrhoeae - cytology
Neisseria gonorrhoeae - growth & development
Time Factors
Uterine Cervical Diseases - microbiology
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Summary:A system for the preliminary evaluation of media to detect Neisseria gonorrhoeae is described. A dissecting microscope fitted with an eyepiece reticulum was used to measure colony diameters at 18 and 22 hours, and clinical trials in a health department venereal disease clinic were used to determine isolation rate efficiencies of media. Measurements of mean colony diameter had good predictive value of media quality in that they were in agreement with isolation rates found in clinical trials. The use of hemoglobin in Thayer-Martin medium yielded greater colony diameters of N gonorrhoeae, and also higher isolation rates in clinical trials. Kellogg's supplement, containing only 4 of the 12 components of commercial supplements and an increased concentration of glucose and iron, performed as well as commercial supplement. Since Kellogg's supplement can be readily prepared in the laboratory, is much less expensive and equally effective, its use in lieu of commercial supplements is recommended.
ISSN:0095-148X
2331-2955