Banded Marker Chromosomes as Indicators of Intraspecies Cellular Contamination

Chromosome banding revealed marker chromosomes characteristic of HeLa cells in cultures designated HEK, HEK/HRV, HBT-3, HBT-39B, MA160, and a strain of SA-4TxS-HuSa$_{1}$. Other HeLa cell characteristics found were glucose-6-phosphate dehydrogenase type A mobility and lack of the Y chromosome. Conve...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1974-06, Vol.184 (4141), p.1093-1096
Main Authors: Nelson-Rees, Walter A., Flandermeyer, Robert R., Hawthorne, Paula K.
Format: Article
Language:English
Subjects:
Analgesia
Cell Line
Cell lines
Chromosomes
Chromosomes - ultrastructure
Cultured cells
Decision theory
Embryonic cells
HeLa cells
HeLa Cells - ultrastructure
Human Y chromosome
Humans
Isochromosomes
Karyotyping
Pain
Sex Chromosomes
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Summary:Chromosome banding revealed marker chromosomes characteristic of HeLa cells in cultures designated HEK, HEK/HRV, HBT-3, HBT-39B, MA160, and a strain of SA-4TxS-HuSa$_{1}$. Other HeLa cell characteristics found were glucose-6-phosphate dehydrogenase type A mobility and lack of the Y chromosome. Conventional chromosome analysis and immunological and enzymatic techniques serve to monitor species specificity and racial origin of the donor. Chromosome banding, however, can monitor intralinear karyotype peculiarity and its evolution during long-term cultivation.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.184.4141.1093