Direct immunofluorescent technique in diagnosis of experimental salmonellosis [Salmonella heidelberg] of turkeys [Bacterial diseases]

Young turkey poults were experimentally infected with Salmonella heidelberg to evaluate the standard tube agglutination test (TAT) and direct fluorescent antibody test (DFAT), and to correlate results of these procedures with the spread pattern of the organisms in the feces using conventional cultur...

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Bibliographic Details
Published in:Avian diseases 1975, Vol.19 (1), p.59-66
Main Authors: Bahl, A.K, Kumar, M.C, York, M.D, Pomeroy, B.S
Format: Article
Language:English
Subjects:
Agglutination Tests
Animals
Antibodies
Cloaca - microbiology
Culture Media
False negative errors
False positive errors
Feces
Feces - microbiology
Fluorescent Antibody Technique
Fluorescent antibody techniques
Poultry Diseases - diagnosis
Poultry Diseases - immunology
Poultry Diseases - microbiology
Poults
Salmonella
Salmonella - growth & development
Salmonella - immunology
Salmonella - isolation & purification
Salmonella enterica subsp. enterica serovar Heidelberg
Salmonella infections
Salmonella Infections, Animal - diagnosis
Salmonella Infections, Animal - immunology
Salmonella Infections, Animal - microbiology
Turkeys
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Summary:Young turkey poults were experimentally infected with Salmonella heidelberg to evaluate the standard tube agglutination test (TAT) and direct fluorescent antibody test (DFAT), and to correlate results of these procedures with the spread pattern of the organisms in the feces using conventional culture procedures for the detection and isolation of S. heidelberg from cloacal swabs. The DFAT test detected fecal excretors considerably longer than bacterial isolation and gave better results. The cultural method was not the most reliable and sensitive method, especially when compared with the DFAT. All control birds remained negative bacteriologically, serologically, and with the DFAT during the observation period.
ISSN:0005-2086
1938-4351
DOI:10.2307/1588955