Organ Cultures of Rat and Hamster Colon

Human fibrin foam as a matrix for three-dimensional organ cultures was successfully employed for the cultivation of newborn rat and hamster colon tissue. Colonic tissue was maintained under different oxygen atmospheres and with various synthetic media. Explants of rat and hamster colon, maintained i...

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Bibliographic Details
Published in:In Vitro 1975-01, Vol.11 (1), p.46-49
Main Author: Schiff, L J
Format: Article
Language:English
Subjects:
Animals
Architecture
Cell Survival
Colon - anatomy & histology
Colon - microbiology
Colon - physiology
Cricetinae
Culture Media
DNA - metabolism
Epithelial Cells
Fibrin Foam
Foams
Human growth
Humans
Mammalian orthoreovirus 3 - growth & development
Organ culture techniques
Organ Culture Techniques - methods
Parainfluenza Virus 1, Human - growth & development
Rats
Reoviridae
Sendai virus
Thymidine - metabolism
Tissue culture techniques
Tritium
Tumors
Viability
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Summary:Human fibrin foam as a matrix for three-dimensional organ cultures was successfully employed for the cultivation of newborn rat and hamster colon tissue. Colonic tissue was maintained under different oxygen atmospheres and with various synthetic media. Explants of rat and hamster colon, maintained in Leibovitz's L-15 medium buffered with HEPES in the presence of 5% CO2and air, retained their normal columnar epithelial architecture for 24 days in culture. Outgrowth of epithelial cells from colonic explants similar to those of the mother fragments grew into the foam. Viability of the explants was indicated by radioactive thymidine incorporation, and by the ability of the fragments to support viral replication.
ISSN:0073-5655
1475-2689
DOI:10.1007/BF02615321