Use of Phenol in the Isolation of Erythropoietic Glycoprotein

THE erythropoietic activity in the plasma of rabbits made anæmic with phenylhydrazine can be isolated as an acid glycoprotein. A dialysed and lyophilized ethanol fraction from heat-coagulated plasma consistently gives us a potent stable highly water-soluble preparation. Six laboratories have found g...

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Bibliographic Details
Published in:Nature (London) 1961-10, Vol.192 (4797), p.75-75
Main Authors: LOWY, PETER H, KEIGHLEY, GEOFFREY
Format: Article
Language:English
Subjects:
Glycoproteins - blood
Hematopoiesis
Humanities and Social Sciences
Humans
letter
multidisciplinary
Old Medline
Phenol
Phenols - chemistry
Science
Science (multidisciplinary)
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Summary:THE erythropoietic activity in the plasma of rabbits made anæmic with phenylhydrazine can be isolated as an acid glycoprotein. A dialysed and lyophilized ethanol fraction from heat-coagulated plasma consistently gives us a potent stable highly water-soluble preparation. Six laboratories have found good correlation in a comparative assay of a batch prepared by us 1 . Further di-ethyl-amino-ethyl cellulose column fractionation removes 40–50 per cent of inert protein and results in the active glycoprotein previously described 2 .
ISSN:0028-0836
1476-4687
DOI:10.1038/192075a0