Structural studies of the capsular polysaccharide of Klebsiella type 28

The capsular polysaccharide from Klebsiella type 28 has been studied by methylation analysis, a modified Smith-degradation procedure, and uronic acid degradation with subsequent oxidation and elimination of the substituents of the oxidized residue. The polysaccharide contained the hexasaccharide rep...

Full description

Saved in:
Bibliographic Details
Published in:Carbohydrate research 1975-06, Vol.42 (1), p.95-105
Main Authors: Curvall, Margareta, Lindberg, Bengt, Lönngren, Jörgen, Nimmich, Wolfgang
Format: Article
Language:English
Subjects:
Chemical Phenomena
Chemistry
Galactose - analysis
Glucose - analysis
Glucuronates - analysis
Klebsiella - analysis
Klebsiella - ultrastructure
Mannose - analysis
Methylation
Molecular Conformation
Optical Rotation
Oxidation-Reduction
Periodic Acid
Polysaccharides, Bacterial - analysis
Subcellular Fractions - analysis
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The capsular polysaccharide from Klebsiella type 28 has been studied by methylation analysis, a modified Smith-degradation procedure, and uronic acid degradation with subsequent oxidation and elimination of the substituents of the oxidized residue. The polysaccharide contained the hexasaccharide repeating-unit shown below. The terminal D-glucopyranose residue was hydrolysed by emulsin, indicating a β linkage. The anomeric natures of other glycosidic linkages were determined by characterization of fragments obtained during the degradative studies. The D-galactopyranose residue was not present in any fragment, but is assumed to be α-linked from optical-rotation considerations. ▪
ISSN:0008-6215
1873-426X
DOI:10.1016/S0008-6215(00)84103-8