The quantitative analysis of uronic acid polymers by infrared spectroscopy

I.r. absorption bands associated with the functional groups of carboxylic acid derivatives are useful for the analysis of alginates and pectins. The ester, amide, and uronate contents of pectins and the uronate content of alginates were determined, respectively, from the ester-carbonyl stretching ba...

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Bibliographic Details
Published in:Carbohydrate research 1975-07, Vol.42 (2), p.217-226
Main Authors: Bociek, Stephen M., Welti, David
Format: Article
Language:English
Subjects:
Alginates - analysis
Deuterium
Methods
Pectins - analysis
Polysaccharides - analysis
Spectrophotometry, Infrared
Uronic Acids - analysis
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Summary:I.r. absorption bands associated with the functional groups of carboxylic acid derivatives are useful for the analysis of alginates and pectins. The ester, amide, and uronate contents of pectins and the uronate content of alginates were determined, respectively, from the ester-carbonyl stretching band (1740 cm −1), the amide I band (1650cm −1), and the carboxylate antisymmetric stretching band (1607cm −1) obtained from the spectra of solutions in D 2O-phosphate buffer. The results are accurate to within ±2-4%, are self consistent, and agree well with the few reliable results that are available. The method should be applicable for the determination of carboxylic acid derivatives in other polysaccharides.
ISSN:0008-6215
1873-426X
DOI:10.1016/S0008-6215(00)84263-9