Polysaccharide production and the possible occurrence of GDP-D-mannose dehydrogenase in Azotobacter vinelandii

During the growth of Azotobacter vinelandii in batch culture in Burk's 2% glucose medium supplemented with 50 mg EDTA per litre, water-insoluble capsular polysaccaride material accumulated in cultures prior to the appearance of water-soluble polysaccharide in the culture medium. On isolation, h...

Full description

Saved in:
Bibliographic Details
Published in:Antonie van Leeuwenhoek 1975-01, Vol.41 (1), p.25-32
Main Authors: Couperwhite, I, McCallum, M F
Format: Article
Language:English
Subjects:
Alcohol Oxidoreductases - metabolism
Alginates - biosynthesis
Azotobacter - metabolism
Chromatography, Paper
Guanosine Diphosphate Mannose - metabolism
Polysaccharides, Bacterial - biosynthesis
Viscosity
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:During the growth of Azotobacter vinelandii in batch culture in Burk's 2% glucose medium supplemented with 50 mg EDTA per litre, water-insoluble capsular polysaccaride material accumulated in cultures prior to the appearance of water-soluble polysaccharide in the culture medium. On isolation, hydrolysis and chromatography, both these polysaccharides were observed to be composed of carbohydrate monomers having the same chromatographic mobilities as glucose, rhamnos, guluronic acid and mannuronic acid. The activity of GDP-D-mannose dehydrogenase recorded in crude cell-free extracts from Azotobacter vinelandii, when these polysaccharides were produced, may indicate a close similarity between the biosynthetic pathway of alginate synthesis in marine Phaeophyceae and this soil microorganism.
ISSN:0003-6072
DOI:10.1007/BF02565034