Use of cationic disc electrophoresis near neutral pH in the evaluation of trace proteins in human plasma

Cationic discontinuous electrophoresis can be carried out at pH 6.0 With excellent resolution in urea-containing acrylamide gels with potassium as the leading ion, 3-picoline as the trailing ion, and cacodylic acid as the buffer. This analytic technique has consistently demonstrated trace protein ab...

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Bibliographic Details
Published in:Cancer research (Chicago, Ill.) Ill.), 1975-08, Vol.35 (8), p.1991-1995
Main Authors: Young, C W, Dessources, W, Hodas, S, Bittar, E S
Format: Article
Language:English
Subjects:
Bacterial Infections - blood
Blood Protein Electrophoresis - methods
Blood Proteins - analysis
Electrophoresis, Disc - methods
Fever - blood
Hodgkin Disease - blood
Humans
Hydrogen-Ion Concentration
Leukemia - blood
Lymphoma - blood
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Summary:Cationic discontinuous electrophoresis can be carried out at pH 6.0 With excellent resolution in urea-containing acrylamide gels with potassium as the leading ion, 3-picoline as the trailing ion, and cacodylic acid as the buffer. This analytic technique has consistently demonstrated trace protein abnormalities in plasma or serum from febrile patients. It has made possible the detection of three protein bands that were obscured in similar electrophoretic assays at pH 3.8 A rough parallelism is found in the plasma (or serum) content of the five protein bands regardless of the apparent clinical cause of the febrile state.
ISSN:0008-5472