Kinetics of conformational changes in tRNA Phe (yeast) as studied by the fluorescence of the y-base and of formycin substituted for the 3'-terminal adenine

The kinetics of the melting transitions of tRNA phe (yeast) were followed by the fluorescence of the Y-base and of formycin substituted for the 3'-terminal adenine. As judged from differential UV absorbance melting cutves the formycin label had virtually no influence on the conformation of the...

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Bibliographic Details
Published in:Biophysical chemistry 1975-10, Vol.3 (4), p.275-289
Main Authors: Coutts, Stephen M., Riesner, Detlev, Römer, Roland, Rabl, Cad R., Maass, Guenter
Format: Article
Language:English
Subjects:
Adenine
Binding Sites
Formycins
Hot Temperature
Kinetics
Mathematics
Nucleic Acid Conformation
Nucleic Acid Denaturation
Phenylalanine
RNA Nucleotidyltransferases
RNA, Transfer
Saccharomyces cerevisiae
Spectrometry, Fluorescence
Spectrophotometry, Ultraviolet
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Summary:The kinetics of the melting transitions of tRNA phe (yeast) were followed by the fluorescence of the Y-base and of formycin substituted for the 3'-terminal adenine. As judged from differential UV absorbance melting cutves the formycin label had virtually no influence on the conformation of the tRNA. A temperature jump apparatus was modified to allow the simultaneous observation of transmission and fluorescence intensities by two independent optical channels. The design of a temperature jump cell with an all quartz center piece is given. The cell is resistant to temperatures up to 90°C; it provides high optical sensitivity, low stray light intensity and the possibility of measuring fluorescence polarization. The T-jump experiments allowed to discriminate between fast unspecific fluorescence quenching (τ
ISSN:0301-4622
1873-4200
DOI:10.1016/0301-4622(75)80020-2