Changing Patterns of Actin Localization during Cell Division

Changing patterns of actin localization have been studied on a light microscopic level by means of fluorescently labeled heavy meromyosin. The cellular distribution of actin is characterized by four major patterns, each of which corresponds to a particular phase of cell division. Long actin fibers a...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1975-05, Vol.72 (5), p.1913-1916
Main Author: Sanger, Joseph W.
Format: Article
Language:English
Subjects:
Actins
Actins - analysis
Actins - metabolism
Animals
Cell Division
Cells
Cells, Cultured
Chick Embryo
Chromatography, Gel
Cytokinesis
Daughter cells
Fibroblasts
Fibroblasts - analysis
Fibroblasts - metabolism
Fibroblasts - ultrastructure
Fluoresceins
Fluorescence
Interphase
Microscopes
Microscopy, Fluorescence
Muscles - metabolism
Myofibrils
Myosin Subfragments - metabolism
Rabbits
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Summary:Changing patterns of actin localization have been studied on a light microscopic level by means of fluorescently labeled heavy meromyosin. The cellular distribution of actin is characterized by four major patterns, each of which corresponds to a particular phase of cell division. Long actin fibers are a prominent feature of the interphase cell. They disappear as the cell rounds up for mitosis and are replaced by a diffuse distribution of actin throughout the cytoplasm. During cytokinesis, the actin is localized predominantly in the cleavage furrow. The final shift of actin occurs after the completion of cytokinesis. At this time the actin becomes concentrated in the distal poles of the cell where pseudopods form to pull the daughter cells apart. When the daughter cells have separated, they flatten on the culture dish and the fibrous pattern of actin characteristic of interphase cells returns. All of these changes take place during the 1-hr period required for cell division.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.72.5.1913