Bacteriological Activity in Unfiltered Calf Sera Collected for Tissue Culture Use

Bacteriological tests were made on 24 lots of unfiltered calf serum collected for subsequent use as a component of tissue culture media. The examination included the isolation and identification of bacteria, assay of phages, and demonstration of endotoxin material. Only Gram-positive bacteria were i...

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Bibliographic Details
Published in:In Vitro 1975-07, Vol.11 (4), p.230-233
Main Authors: Orr, Henry C., Sibinovic, Kyle H., Peter G. Probst, Hochstein, H. Donald, Dewitt C. Littlejohn
Format: Article
Language:English
Subjects:
Bacillus
Bacillus - isolation & purification
Bacteria
Bacteria - isolation & purification
Bacterial flora
Bacteriophages
Bacteriophages - isolation & purification
Blood
Blood - microbiology
Body tissues
Cell culture techniques
Culture Media
Endotoxins
Staphylococcus - isolation & purification
Streptococcus - isolation & purification
Tissue culture techniques
Ungulates
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Summary:Bacteriological tests were made on 24 lots of unfiltered calf serum collected for subsequent use as a component of tissue culture media. The examination included the isolation and identification of bacteria, assay of phages, and demonstration of endotoxin material. Only Gram-positive bacteria were isolated and 96% of the sera were contaminated with bacteria. The prevalent strains of bacteria found were Bacillus species and streptococci and 63% of the sera coagulated Limulus amebocyte lysate. More than 90% of the lots contained phages demonstrable with the C-3000 strain of Escherichia coli. Only one lot of the serum was found to be free from bacteria, phages, and endotoxin by the tests used.
ISSN:0073-5655
1475-2689
DOI:10.1007/BF02616338