influence of pH on the growth and stability of transmissible gastroenteritis virus in vitro

The influence of pH on the growth of transmissible gastroenteritis virus (TGEV) in adult pig thyroid cell culture, and on the stability of the virus was studied. At pH 7.2 and 100 fold higher than those at pH 8.0. The adsorption, penetration and uncoating steps of the viral replicative cycle were sh...

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Bibliographic Details
Published in:Archives of virology 1975, Vol.49 (2/3), p.239-247
Main Authors: Pocock, D.H, Garwes, D.J
Format: Article
Language:English
Subjects:
Animals
Cell Line
Cells, Cultured
Coronaviridae - growth & development
Culture Media
HEPES - pharmacology
Hot Temperature
Hydrogen-Ion Concentration
RNA, Viral - biosynthesis
Swine
Thyroid Gland
Transmissible gastroenteritis virus - growth & development
Transmissible gastroenteritis virus - immunology
Transmissible gastroenteritis virus - metabolism
Virus Replication - drug effects
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Summary:The influence of pH on the growth of transmissible gastroenteritis virus (TGEV) in adult pig thyroid cell culture, and on the stability of the virus was studied. At pH 7.2 and 100 fold higher than those at pH 8.0. The adsorption, penetration and uncoating steps of the viral replicative cycle were shown to be unaffected by pH variation. Synthesis of TGEV RNA during the first 12 hours post infection was found to be unaffected by pH variation between the range 6.5-8.0. After 12 hours breakdown of this RNA appeared to occur in cultures held at pH 7.2 and 8.0 but not at pH 6.5. When incubated at 37 degrees C for 24 hours the virus infectivity was found to be least affected by pH 6.5 but when kept at 4 degrees C for the same length of time, the virus infectivity remained constant between pH 5.0 and pH 8.0.
ISSN:0304-8608
1432-8798
DOI:10.1007/BF01317542