Reactivity of Smooth-Muscle Antibodies, Surface Ultrastructure, and Mobility in Cells of Human Hematopoietic Cell Lines

Seventeen human hematopoietic cell lines were tested by indirect immunofluorescence (IF) for reactivity with human serum containing smooth-muscle antibodies (SMA). The correlation of the IF pattern to the cell surface ultrastructure was revealed by scanning electron microscopy (SEM). Lymphoma cells,...

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Bibliographic Details
Published in:JNCI : Journal of the National Cancer Institute 1975-10, Vol.55 (4), p.783-789
Main Authors: Fagraeus, Astrid, Nilsson, Kenneth, Knut, Lidman, Norberg, Renée
Format: Article
Language:English
Subjects:
Antibodies
Cell Differentiation
Cell Line
Cell Movement
Hematopoietic System - cytology
Leukemia - immunology
Leukemia - pathology
Lymphocytes - cytology
Lymphocytes - immunology
Lymphoma - immunology
Lymphoma - pathology
Multiple Myeloma - immunology
Multiple Myeloma - pathology
Muscle, Smooth - immunology
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Summary:Seventeen human hematopoietic cell lines were tested by indirect immunofluorescence (IF) for reactivity with human serum containing smooth-muscle antibodies (SMA). The correlation of the IF pattern to the cell surface ultrastructure was revealed by scanning electron microscopy (SEM). Lymphoma cells, viewed by SEM, had short villi over the entire cell surface, but, by IF, showed a type of membrane fluorescence. Cells of Iymphoblastoid lines had thin, long, surface villi, sometimes asymmetric but most often distributed over the whole cell surface. Myeloma and leukemia cells, which had few membrane villi but a surface covered by “blebs” as revealed by SEM, demonstrated, by IF, only a few stub-like projections extending from the surface. Time-lapse cinematography revealed that the intensity and pattern of SMA staining were also correlated to the degree of motility. Indirect IF with human SMA-positive serum might be used in the classification of cell lines derived from human hematopoietic tissue.
ISSN:0027-8874
1460-2105
DOI:10.1093/jnci/55.4.783