Replacing a reservoir solution with desiccant in vapor diffusion protein crystallization screening

This paper presents a modification to the conventional vapor diffusion (hanging‐ or sitting‐drop) technique for protein crystallization screening. In this modified method, the reservoir solution is replaced with a desiccant to allow for a larger range of protein solution concentrations, thereby prov...

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Bibliographic Details
Published in:Journal of applied crystallography 2010-10, Vol.43 (5-1), p.1021-1026
Main Authors: Lu, Qin-Qin, Yin, Da-Chuan, Chen, Rui-Qing, Xie, Si-Xiao, Liu, Yong-Ming, Zhang, Xian-Fang, Zhu, Li, Liu, Zheng-Tang, Shang, Peng
Format: Article
Language:English
Subjects:
Cost engineering
Crystallization
crystallization screening
Crystals
Desiccants
Diffusion
Efficiency
protein crystallization
Proteins
Reproducibility
Research methodology
Reservoirs
Screening
vapor diffusion
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Summary:This paper presents a modification to the conventional vapor diffusion (hanging‐ or sitting‐drop) technique for protein crystallization screening. In this modified method, the reservoir solution is replaced with a desiccant to allow for a larger range of protein solution concentrations, thereby providing more opportunities for crystal formation. This method was tested in both reproducibility and screening studies, and the results showed that it significantly improves the efficiency and reduces the cost of protein crystallization screens.
ISSN:1600-5767
0021-8898
1600-5767
DOI:10.1107/S0021889810030803