a4b1 and a5b1 Control Cell Migration on Fibronectin by Differentially Regulating Cell Speed and Motile Cell Phenotype

• Published in: Annals of biomedical engineering 1998-11, Vol.26 (6), p.1091-1101
• Main Authors: Chon, John H, Netzel, Rivka, Rock, Barbara M, Chaikof, Elliot L
• Format: Article
• Language: English
• ISSN: 0090-6964; 1573-9686
• DOI: 10.1114/1.139

Computer-aided time lapse fluorescence videomicroscopy was used to study single cell migration behavior of human aortic endothelial cells on fibronectin coated substrates of varying protein surface density. The role of receptors a _ sub(5b) _ sub(1), a_ sub(vb) _ sub(3), and a sub(4)b sub(1) in mediating cell adhesion and migration on fibronectin was characterized using integrin specific monoclonal antibodies. Matrix density had a direct effect on controlling the proportion of migrating cells and the directional persistence of cell movement (p < 0.01). While there was relatively little influence of fibronectin surface density on absolute migration speed, the ability of endothelial cells to disperse over a surface, as measured by the dispersion coefficient, was biphasic with respect to the surface density of this matrix protein (p < 0.005). Both cell speed and the proportion of migrating cells was controlled by a sub(4)b sub(1) (p < 0.01). However, a sub(5)b sub(1) selectively regulated the transformation of stationary cells to those exhibiting motile behavior (p