A plasma global metabolic profiling approach applied to an exercise study monitoring the effects of glucose, galactose and fructose drinks during post-exercise recovery

A global metabolic profiling methodology based on gas chromatography coupled to time-of-flight mass spectrometry (GC–TOFMS) for human plasma was applied to a human exercise study focused on the effects of beverages containing glucose, galactose, or fructose taken after exercise and throughout a reco...

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Bibliographic Details
Published in:Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2010-11, Vol.878 (29), p.3015-3023
Main Authors: Bruce, Stephen J., Breton, Isabelle, Decombaz, Jacques, Boesch, Chris, Scheurer, Eva, Montoliu, Ivan, Rezzi, Serge, Kochhar, Sunil, Guy, Philippe A.
Format: Article
Language:English
Subjects:
Adult
Analysis
Analytical, structural and metabolic biochemistry
Beverages
Beverages - analysis
Biological and medical sciences
Blood Glucose - analysis
Consumption
Exercise
Fatty Acids - blood
Fatty Acids - metabolism
Fructose
Fructose - blood
Fructose - metabolism
Fundamental and applied biological sciences. Psychology
Galactonic acid
Galactose
Galactose - blood
Galactose - metabolism
GC-TOF
General pharmacology
Glucose
Glucose - metabolism
Human
Human nutrition
Humans
Insulin - blood
Insulin - metabolism
Male
Medical sciences
Metabolome
Metabolomics
Pharmacology. Drug treatments
Post-exercise recovery
Profiling
Recovery
Sports drink
Young Adult
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Summary:A global metabolic profiling methodology based on gas chromatography coupled to time-of-flight mass spectrometry (GC–TOFMS) for human plasma was applied to a human exercise study focused on the effects of beverages containing glucose, galactose, or fructose taken after exercise and throughout a recovery period of 6 h and 45 min. One group of 10 well trained male cyclists performed 3 experimental sessions on separate days (randomized, single center). After performing a standardized depletion protocol on a bicycle, subjects consumed one of three different beverages: maltodextrin (MD) + glucose (2:1 ratio), MD + galactose (2:1), and MD + fructose (2:1), consumed at an average of ∼1.25 g of carbohydrate (CHO) ingested per minute. Blood was taken straight after exercise and every 45 min within the recovery phase. With the resulting blood plasma, insulin, free fatty acid (FFA) profile, glucose, and GC–TOFMS global metabolic profiling measurements were performed. The resulting profiling data was able to match the results obtained from the other clinical measurements with the addition of being able to follow many different metabolites throughout the recovery period. The data quality was assessed, with all the labelled internal standards yielding values of
ISSN:1570-0232
1873-376X
DOI:10.1016/j.jchromb.2010.09.004