New and Expedient Determination of Atenolol in Biological Samples

A method is described for the GLC determination of atenolol BP in plasma and urine. Extraction is accomplished under dehydrating conditions, and interfering impurities are removed by using an acidified cyclohexane‐isopropanol mixture (2:1) and charcoal‐treated paper disks. The drug thus isolated app...

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Bibliographic Details
Published in:Journal of pharmaceutical sciences 1975-12, Vol.64 (12), p.1992-1994
Main Authors: Malbica, J.O., Monson, K.R.
Format: Article
Language:English
Subjects:
Adrenergic agents-atenolol
Adrenergic agents—atenolol, GLC analysis, biological samples
Adrenergic beta-Antagonists - analysis
Adrenergic beta-Antagonists - blood
Adrenergic beta-Antagonists - urine
Adult
Atenolol
Atenolol-GLC analysis
Atenolol—GLC analysis, biological samples
biological samples
Chromatography, Gas
GLC analysis
GLC—analysis, atenolol, biological samples
Humans
Male
Methods
Time Factors
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Summary:A method is described for the GLC determination of atenolol BP in plasma and urine. Extraction is accomplished under dehydrating conditions, and interfering impurities are removed by using an acidified cyclohexane‐isopropanol mixture (2:1) and charcoal‐treated paper disks. The drug thus isolated appears to react more efficiently with heptafluorobutyric anhydride, increasing the sensitivity of GLC electron‐capture analysis. Concentrations as low as 0.02 μg/ml were measured using 0.5‐ml aliquots of plasma or 0.1ml of urine. Amino alcohols such as atenolol may form hydrates or alcoholates, precluding complete derivatization with heptafluorobutyric anhydride.
ISSN:0022-3549
1520-6017
DOI:10.1002/jps.2600641219