The Stimulatory Effect of the Boiled Supernatant on Cyclic AMP Formation in Synaptosomes from Rat Cerebral Cortex

The effect of the boiled supernatant on synaptosomal adenyl cyclase activity of rat cerebral cortex was investigated. The boiled supernatant markedly increased the accumulation of cyclic AMP in synaptosomes when added to adenyl cyclase system by a mechanism presumably unrelated to inhibition of cycl...

Full description

Saved in:
Bibliographic Details
Published in:Japanese journal of pharmacology 1975, Vol.25(4), pp.375-381
Main Authors: Izumi, Hiroshi, Oyama, Hideko, Ozawa, Hikaru
Format: Article
Language:English
Subjects:
3',5'-Cyclic-AMP Phosphodiesterases - metabolism
Adenosine Triphosphatases - metabolism
Adenosine Triphosphate - metabolism
Adenylyl Cyclases - metabolism
Animals
Cerebral Cortex - metabolism
Dopamine - pharmacology
Fluorides - pharmacology
Histamine - pharmacology
Hot Temperature
Magnesium - pharmacology
Male
Rats
Serotonin - pharmacology
Synaptosomes - enzymology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The effect of the boiled supernatant on synaptosomal adenyl cyclase activity of rat cerebral cortex was investigated. The boiled supernatant markedly increased the accumulation of cyclic AMP in synaptosomes when added to adenyl cyclase system by a mechanism presumably unrelated to inhibition of cyclic AMP phosphodiesterase or adenosine triphosphatase. Magnesium ion was required for synaptosomal adenyl cyclase activity and its stimulation by the boiled supernatant. The result discerned by double reciprocal plots showed an increase in Vmax value of adenyl cyclase by addition of the boiled supernatant without significantly altering the affinity for substrate. The enzyme activity was not stimulated by dopamine, histamine and serotonin in either the absence and presence of the boiled supernatant.
ISSN:0021-5198
1347-3506
DOI:10.1254/jjp.25.375