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The Stimulatory Effect of the Boiled Supernatant on Cyclic AMP Formation in Synaptosomes from Rat Cerebral Cortex

The effect of the boiled supernatant on synaptosomal adenyl cyclase activity of rat cerebral cortex was investigated. The boiled supernatant markedly increased the accumulation of cyclic AMP in synaptosomes when added to adenyl cyclase system by a mechanism presumably unrelated to inhibition of cycl...

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Published in:	Japanese journal of pharmacology 1975, Vol.25(4), pp.375-381
Main Authors:	Izumi, Hiroshi, Oyama, Hideko, Ozawa, Hikaru
Format:	Article
Language:	English
Subjects:	3',5'-Cyclic-AMP Phosphodiesterases - metabolism Adenosine Triphosphatases - metabolism Adenosine Triphosphate - metabolism Adenylyl Cyclases - metabolism Animals Cerebral Cortex - metabolism Dopamine - pharmacology Fluorides - pharmacology Histamine - pharmacology Hot Temperature Magnesium - pharmacology Male Rats Serotonin - pharmacology Synaptosomes - enzymology
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creator	Izumi, Hiroshi Oyama, Hideko Ozawa, Hikaru
description	The effect of the boiled supernatant on synaptosomal adenyl cyclase activity of rat cerebral cortex was investigated. The boiled supernatant markedly increased the accumulation of cyclic AMP in synaptosomes when added to adenyl cyclase system by a mechanism presumably unrelated to inhibition of cyclic AMP phosphodiesterase or adenosine triphosphatase. Magnesium ion was required for synaptosomal adenyl cyclase activity and its stimulation by the boiled supernatant. The result discerned by double reciprocal plots showed an increase in Vmax value of adenyl cyclase by addition of the boiled supernatant without significantly altering the affinity for substrate. The enzyme activity was not stimulated by dopamine, histamine and serotonin in either the absence and presence of the boiled supernatant.
doi_str_mv	10.1254/jjp.25.375
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The boiled supernatant markedly increased the accumulation of cyclic AMP in synaptosomes when added to adenyl cyclase system by a mechanism presumably unrelated to inhibition of cyclic AMP phosphodiesterase or adenosine triphosphatase. Magnesium ion was required for synaptosomal adenyl cyclase activity and its stimulation by the boiled supernatant. The result discerned by double reciprocal plots showed an increase in Vmax value of adenyl cyclase by addition of the boiled supernatant without significantly altering the affinity for substrate. 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subjects	3',5'-Cyclic-AMP Phosphodiesterases - metabolism Adenosine Triphosphatases - metabolism Adenosine Triphosphate - metabolism Adenylyl Cyclases - metabolism Animals Cerebral Cortex - metabolism Dopamine - pharmacology Fluorides - pharmacology Histamine - pharmacology Hot Temperature Magnesium - pharmacology Male Rats Serotonin - pharmacology Synaptosomes - enzymology
title	The Stimulatory Effect of the Boiled Supernatant on Cyclic AMP Formation in Synaptosomes from Rat Cerebral Cortex
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