Isolation and Characterization of Calcitonin from Pericardium and Esophagus of Eel

Calcitonin was extracted from the pericardium and esophagus of eel in quantities sufficient to permit purification and chemical characterization. Homogeneous calcitonin could be isolated by a six-step fractionation starting from acetone powder of the organs. The fractionation procedure consisted of...

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Bibliographic Details
Published in:Journal of biochemistry (Tokyo) 1976-02, Vol.79 (2), p.345-352
Main Authors: OTANI, Masaru, YAMAUCHI, Hirose, MEGURO, Toshio, KITAZAWA, Shyuichi, WATANABE, Susumu, ORIMO, Hajime
Format: Article
Language:English
Subjects:
Amino Acids - analysis
Animals
Calcitonin - isolation & purification
Calcitonin - pharmacology
Calcium - blood
Chromatography, Gel
Eels
Esophagus - analysis
Molecular Weight
Pericardium - analysis
Rats
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Summary:Calcitonin was extracted from the pericardium and esophagus of eel in quantities sufficient to permit purification and chemical characterization. Homogeneous calcitonin could be isolated by a six-step fractionation starting from acetone powder of the organs. The fractionation procedure consisted of acid extraction, gel filtration on Sephadex G-75, chromatography on SP-Sephadex C-25, gel filtration on Sephadex G-50, chroma-tography on carboxymethylcellulose, and gel filtration on Sephadex G-50. Fractionation of the hormone was monitored by assay of its biological activity and from its behaviour on thin layer chromatography and polyacrylamide gel disc electrophoresis. The hormone contained 32 amino acid residues, like calcitonins from other species of animals, but its amino acid composition was different from those of previously characterized hormones. Eel calcitonin possessed almost the same, or higher, biological activity as the salmon or chicken hormone, which show the highest specific activity among calcitonins so far isolated.
ISSN:0021-924X
1756-2651
DOI:10.1093/oxfordjournals.jbchem.a131077