incorporation of nitrogen into products of recent photosynthesis in Anabaena cylindrica Lemm

In vivo tracer studies with 14C have been performed to help determine pathways of incorporation of newly assimilated nitrogen into N2-fixing cells of Anabaena cylindrica. After photosynthesis in Ar: O2:14CO2 for 30 min, the addition of N2 or NH+4 resulted in increased rates of 14CO2-incorporation bo...

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Bibliographic Details
Published in:Archives of microbiology 1976-02, Vol.107 (1), p.15-24
Main Authors: Lawrie, A.C, Codd, G.A, Stewart, W.D.P
Format: Article
Language:English
Subjects:
Alanine - biosynthesis
Algae
Aspartic Acid - biosynthesis
Citric Acid Cycle
Cyanobacteria - metabolism
Darkness
Glutamates - biosynthesis
Glutamine - biosynthesis
Glycine - biosynthesis
Nitrogen - metabolism
Nitrogen Fixation
Photosynthesis
Quaternary Ammonium Compounds - metabolism
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Summary:In vivo tracer studies with 14C have been performed to help determine pathways of incorporation of newly assimilated nitrogen into N2-fixing cells of Anabaena cylindrica. After photosynthesis in Ar: O2:14CO2 for 30 min, the addition of N2 or NH+4 resulted in increased rates of 14CO2-incorporation both in the light and dark, and in increased incorporation of 14C into amino acids at the expense of sucrose and sugar phosphates. Evidence of enhanced sucrose catabolism and increased pyruvate kinase activity was obtained on adding nitrogen, and, of the 14C-labelling entering the tricarboxylic acid cycle, more appeared in citrate and 2-oxoglutarate than in malate and oxaloacetate. The kinetics of 14C-incorporation into various amino acids suggest that in the light and dark the most important route of primary ammonia assimilation involves glutamine synthetase and that glutamate, aspartate, glycine and probably alanine are formed secondarily from glutamine.
ISSN:0302-8933
1432-072X
DOI:10.1007/BF00427862