Establishment of a human carcinoembryonic antigen-producing colon adenocarcinoma cell line

A human carcinoembryonic antigen-producing colon carcinoma cell line has been established. The cells form acinar structures and signet ring cells. The lumen of the acini presents microvilli and a glycocalyx. Neighboring cells show desmosomes and terminal bars. The cells present an aneuploid karyotyp...

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Bibliographic Details
Published in:Cancer research (Chicago, Ill.) Ill.), 1976-02, Vol.36 (2 Pt 1), p.467-475
Main Authors: Drewinko, B, Romsdahl, M M, Yang, L Y, Ahearn, M J, Trujillo, J M
Format: Article
Language:English
Subjects:
Adenocarcinoma - genetics
Adenocarcinoma - immunology
Adenocarcinoma - pathology
Carcinoembryonic Antigen
Cell Division
Cell Line
Cell Survival
Chromosome Aberrations
Chromosomes, Human, 1-3
Chromosomes, Human, 4-5
Chromosomes, Human, 6-12 and X
Colonic Neoplasms - genetics
Colonic Neoplasms - immunology
Colonic Neoplasms - pathology
Humans
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Summary:A human carcinoembryonic antigen-producing colon carcinoma cell line has been established. The cells form acinar structures and signet ring cells. The lumen of the acini presents microvilli and a glycocalyx. Neighboring cells show desmosomes and terminal bars. The cells present an aneuploid karyotype with a modal number of 49. No marker chromosomes are found, although a significant proportion of cells show an altered A2 chromosome and an extra B. Exponentially growing cultures produce 54 ng of carcinoembryonic antigen/10(6) cells. Kinetic parameters are as follows: doubling time, 37 hr; mitotic index, 0.8%; labeling index, 31%; generation time, 30 hr; G1 phase, 7 hr; S phase, 18 hr; G2 phase, 5 hr; growth fraction 90%. This cell line, designated line LoVo, represents an in vitro model for human colon carcinoma.
ISSN:0008-5472