Calcium-binding of synaptosomes isolated from rat brain cortex. IV. Effects of ruthenium red on the Co-operative nature of calcium-binding

Ruthenium red combines with isolated synaptosomes, resulting in strong inhibition of their Ca2+-binding. In isotonic saline media, however, the dye-induced inhibition of Ca2+-binding is significantly greater than that expected for the amount of bound dye and Hill's exponent of the Ca2+-binding...

Full description

Saved in:
Bibliographic Details
Published in:The Journal of membrane biology 1976-05, Vol.26 (4), p.345-356
Main Authors: Kamino, K, Ogawa, M, Uyesaka, N, Inouye, A
Format: Article
Language:English
Subjects:
Animals
Binding Sites
Calcium - metabolism
Cerebral Cortex - drug effects
Cerebral Cortex - metabolism
Kinetics
Mathematics
Rats
Ruthenium - pharmacology
Ruthenium Red - pharmacology
Spectrophotometry
Synaptosomes - drug effects
Synaptosomes - metabolism
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Ruthenium red combines with isolated synaptosomes, resulting in strong inhibition of their Ca2+-binding. In isotonic saline media, however, the dye-induced inhibition of Ca2+-binding is significantly greater than that expected for the amount of bound dye and Hill's exponent of the Ca2+-binding decreases to 1 with an increase in the amount of the dye bound. On the other hand in isotonic mannitol-sucrose solution, inhibition of synaptosmal Ca2+-binding brought about by the dye is proportional to the amount of dye bound. Based on these results, the effects of the dye on the co-operative nature of synaptosomal Ca2+-binding is discussed.
ISSN:0022-2631