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Calcium-binding of synaptosomes isolated from rat brain cortex. IV. Effects of ruthenium red on the Co-operative nature of calcium-binding
Ruthenium red combines with isolated synaptosomes, resulting in strong inhibition of their Ca2+-binding. In isotonic saline media, however, the dye-induced inhibition of Ca2+-binding is significantly greater than that expected for the amount of bound dye and Hill's exponent of the Ca2+-binding...
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| Published in: | The Journal of membrane biology 1976-05, Vol.26 (4), p.345-356 |
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| Main Authors: | , , , |
| Format: | Article |
| Language: | English |
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| container_end_page | 356 |
| container_issue | 4 |
| container_start_page | 345 |
| container_title | The Journal of membrane biology |
| container_volume | 26 |
| creator | Kamino, K Ogawa, M Uyesaka, N Inouye, A |
| description | Ruthenium red combines with isolated synaptosomes, resulting in strong inhibition of their Ca2+-binding. In isotonic saline media, however, the dye-induced inhibition of Ca2+-binding is significantly greater than that expected for the amount of bound dye and Hill's exponent of the Ca2+-binding decreases to 1 with an increase in the amount of the dye bound. On the other hand in isotonic mannitol-sucrose solution, inhibition of synaptosmal Ca2+-binding brought about by the dye is proportional to the amount of dye bound. Based on these results, the effects of the dye on the co-operative nature of synaptosomal Ca2+-binding is discussed. |
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Based on these results, the effects of the dye on the co-operative nature of synaptosomal Ca2+-binding is discussed.</description><identifier>ISSN: 0022-2631</identifier><identifier>PMID: 933150</identifier><language>eng</language><publisher>United States</publisher><subject>Animals ; Binding Sites ; Calcium - metabolism ; Cerebral Cortex - drug effects ; Cerebral Cortex - metabolism ; Kinetics ; Mathematics ; Rats ; Ruthenium - pharmacology ; Ruthenium Red - pharmacology ; Spectrophotometry ; Synaptosomes - drug effects ; Synaptosomes - metabolism</subject><ispartof>The Journal of membrane biology, 1976-05, Vol.26 (4), p.345-356</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/933150$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kamino, K</creatorcontrib><creatorcontrib>Ogawa, M</creatorcontrib><creatorcontrib>Uyesaka, N</creatorcontrib><creatorcontrib>Inouye, A</creatorcontrib><title>Calcium-binding of synaptosomes isolated from rat brain cortex. IV. Effects of ruthenium red on the Co-operative nature of calcium-binding</title><title>The Journal of membrane biology</title><addtitle>J Membr Biol</addtitle><description>Ruthenium red combines with isolated synaptosomes, resulting in strong inhibition of their Ca2+-binding. In isotonic saline media, however, the dye-induced inhibition of Ca2+-binding is significantly greater than that expected for the amount of bound dye and Hill's exponent of the Ca2+-binding decreases to 1 with an increase in the amount of the dye bound. On the other hand in isotonic mannitol-sucrose solution, inhibition of synaptosmal Ca2+-binding brought about by the dye is proportional to the amount of dye bound. Based on these results, the effects of the dye on the co-operative nature of synaptosomal Ca2+-binding is discussed.</description><subject>Animals</subject><subject>Binding Sites</subject><subject>Calcium - metabolism</subject><subject>Cerebral Cortex - drug effects</subject><subject>Cerebral Cortex - metabolism</subject><subject>Kinetics</subject><subject>Mathematics</subject><subject>Rats</subject><subject>Ruthenium - pharmacology</subject><subject>Ruthenium Red - pharmacology</subject><subject>Spectrophotometry</subject><subject>Synaptosomes - drug effects</subject><subject>Synaptosomes - metabolism</subject><issn>0022-2631</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1976</creationdate><recordtype>article</recordtype><recordid>eNpVkL1OwzAUhT3wVwpvwOCJLdV1nJ96RFGBSpVYKtbIsa_BKLGD7SD6Cjw1qcrCdHWk7ztHumdkAZDnWV5xdkWuY_wAYHVdFZfkQnDOSliQn0b2yk5D1lmnrXuj3tB4cHJMPvoBI7XR9zKhpib4gQaZaBekdVT5kPB7RbevK7oxBlWKRzdM6R3dXEjD7HhH50gbn_kRZ9d-IXUyTQGPrPo_fUPOjewj3v7dJdk_bvbNc7Z7edo2D7tsLHPIhNalKNYGQfMyLzjjVVdjJRkiE7zojFRMF0oglHXFQeRQK7Y2jEOpVNUpviT3p9ox-M8JY2oHGxX2vXTop9iuOS8AQMzg3R84dQPqdgx2kOHQnl7HfwEdS2rr</recordid><startdate>197605</startdate><enddate>197605</enddate><creator>Kamino, K</creator><creator>Ogawa, M</creator><creator>Uyesaka, N</creator><creator>Inouye, A</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>197605</creationdate><title>Calcium-binding of synaptosomes isolated from rat brain cortex. 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IV. Effects of ruthenium red on the Co-operative nature of calcium-binding</atitle><jtitle>The Journal of membrane biology</jtitle><addtitle>J Membr Biol</addtitle><date>1976-05</date><risdate>1976</risdate><volume>26</volume><issue>4</issue><spage>345</spage><epage>356</epage><pages>345-356</pages><issn>0022-2631</issn><abstract>Ruthenium red combines with isolated synaptosomes, resulting in strong inhibition of their Ca2+-binding. In isotonic saline media, however, the dye-induced inhibition of Ca2+-binding is significantly greater than that expected for the amount of bound dye and Hill's exponent of the Ca2+-binding decreases to 1 with an increase in the amount of the dye bound. On the other hand in isotonic mannitol-sucrose solution, inhibition of synaptosmal Ca2+-binding brought about by the dye is proportional to the amount of dye bound. Based on these results, the effects of the dye on the co-operative nature of synaptosomal Ca2+-binding is discussed.</abstract><cop>United States</cop><pmid>933150</pmid><tpages>12</tpages></addata></record> |
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| identifier | ISSN: 0022-2631 |
| ispartof | The Journal of membrane biology, 1976-05, Vol.26 (4), p.345-356 |
| issn | 0022-2631 |
| language | eng |
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| source | Springer Nature - Connect here FIRST to enable access |
| subjects | Animals Binding Sites Calcium - metabolism Cerebral Cortex - drug effects Cerebral Cortex - metabolism Kinetics Mathematics Rats Ruthenium - pharmacology Ruthenium Red - pharmacology Spectrophotometry Synaptosomes - drug effects Synaptosomes - metabolism |
| title | Calcium-binding of synaptosomes isolated from rat brain cortex. IV. Effects of ruthenium red on the Co-operative nature of calcium-binding |
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