Antibodies to Histones and Histone-Histone Complexes: Immunochemical Evidence for Secondary Structure in Histone 1

Highly specific antibodies were raised to histone 1 (H1) and the histone complexes H3$_{2}$-H4$_{2}$ and H2A-H2B, isolated by salt extraction. Antibody to H1 could detect irreversible conformational changes in acid- or urea-treated H1. The antibodies showed different reactivities with chromosomes as...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1976-04, Vol.192 (4238), p.469-471
Main Authors: Mihalakis, Nicholas, Miller, Orlando J., Erlanger, Bernard F.
Format: Article
Language:English
Subjects:
Antibodies
Antibody Specificity
Antigens
Antiserum
Cell lines
Chromosomes
Gels
Histones
Histones - immunology
Histones - isolation & purification
Hydrogen-Ion Concentration
Nerves
Neuroglia
Neurons
Urea
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Summary:Highly specific antibodies were raised to histone 1 (H1) and the histone complexes H3$_{2}$-H4$_{2}$ and H2A-H2B, isolated by salt extraction. Antibody to H1 could detect irreversible conformational changes in acid- or urea-treated H1. The antibodies showed different reactivities with chromosomes as compared to antibodies in acid-extracted histones and should be useful in studies of native chromatin and chromosome structure.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.3853