Isolation and characterization of an antigen of the bovine C-type virus

By means of gel filtration and isoelectric focusing, an antigen of the bovine C-type leukemia virus was isolated in a highly purified form from extracts of infected cells. The antigen has a molecular weight of approximately 25,000 daltons and an isoelectric point of 6.4 to 6.6. In immunodiffusion ex...

Full description

Saved in:
Bibliographic Details
Published in:Cancer research (Chicago, Ill.) Ill.), 1976-04, Vol.36 (4), p.1251-1257
Main Authors: McDonald, H C, Graves, D C, Ferrer, J F
Format: Article
Language:English
Subjects:
Antigens, Viral - isolation & purification
Cells, Cultured
Chromatography, Gel
Cross Reactions
Epitopes
Immunodiffusion
Isoelectric Focusing
Isotope Labeling
Leukemia Virus, Bovine - immunology
Leukemia Virus, Feline - immunology
Molecular Weight
Oncogenic Viruses - immunology
Retroviridae - immunology
Viral Proteins - analysis
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:By means of gel filtration and isoelectric focusing, an antigen of the bovine C-type leukemia virus was isolated in a highly purified form from extracts of infected cells. The antigen has a molecular weight of approximately 25,000 daltons and an isoelectric point of 6.4 to 6.6. In immunodiffusion experiments, the antigen forms a line of identity with an antigen extracted from highly purified bovine C-type leukemia virus by treatment with ether or Triton X-100. As determined by immunodiffusion analyses, the bovine C-type leukemia virus antigen does not have antigenic determinants in common with the murine or feline leukemia viruses, the foamy-like bovine syncytia virus, or the Mason Pfizer monkey virus.
ISSN:0008-5472