Binding of Sulfisoxazole to Protein Fractions of Tears

• Published in: Journal of pharmaceutical sciences 1976-03, Vol.65 (3), p.437-439
• Main Authors: Chrai, Sukhbir S., Robinson, Joseph R.
• Format: Article
• Language: English
• Subjects: Albumins - metabolism; binding of sulfisoxazole to protein fractions; Dialysis; Globulins - metabolism; Humans; In Vitro Techniques; Lacrimal fluids-human; Lacrimal fluids—human, normal and irritant induced, binding of sulfisoxazole to protein fractions; Muramidase - metabolism; normal and irritant induced; Protein Binding; Protein binding-sulfisoxazole to protein fractions of human tears; Protein binding—sulfisoxazole to protein fractions of human tears, normal and irritant induced; Sulfisoxazole - metabolism; Sulfisoxazole-binding to protein fractions of human tears; Sulfisoxazole—binding to protein fractions of human tears, normal and irritant induced; Tears - metabolism
• ISSN: 0022-3549; 1520-6017
• DOI: 10.1002/jps.2600650333

By using equilibrium dialysis experiments, it is shown that sulfisoxazole binds extensively with albumin and α‐globulin and to a lesser extent with γ‐globulin and lysozyme, all of which are normal components of tears. The binding of sulfisoxazole to the various protein fractions quantitatively accounts for the overall binding of this drug to human tears. It is suggested that to predict the binding of drugs to proteins in lacrimal fluid, both in normal and pathological conditions, it is necessary to conduct binding studies of the drug with the major protein fractions of tears. Moreover, it is essential to know the composition of the major protein fractions of tears in both normal and various pathological conditions to conduct the necessary in vitro binding studies.