Characterization of an established cell line (SH‐3) derived from pleural effusion of patient with breast cancer

Spontaneous emergence of fast‐growing cells in prolonged culture of pleural effusion cells obtained from a patient with breast cancer has led to the establishment of a cell line designated as SH‐3. By morphological criteria, as revealed by light and electron microscopy, SH‐3 cells are epithelial and...

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Bibliographic Details
Published in:Cancer 1976-04, Vol.37 (4), p.1814-1824
Main Authors: Seman, G., Hunter, S. J., Miller, R. C., Dmochowski, L.
Format: Article
Language:English
Subjects:
Alkaline Phosphatase - metabolism
Breast Neoplasms - enzymology
Breast Neoplasms - genetics
Cell Line
Chromosomes
Glucosephosphate Dehydrogenase - metabolism
Isoenzymes - metabolism
Pleural Effusion - cytology
Polyploidy
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Summary:Spontaneous emergence of fast‐growing cells in prolonged culture of pleural effusion cells obtained from a patient with breast cancer has led to the establishment of a cell line designated as SH‐3. By morphological criteria, as revealed by light and electron microscopy, SH‐3 cells are epithelial and resemble the poorly differentiated cells of a series of established human tumor cell lines. Their karyotype is hypotetraploid and different from that of HeLa cells. Isoenzyme analysis has shown the presence in the cells of a bone‐type alkaline phosphatase and of the fast (A) band of G6PD. The line is free of mycoplasma.
ISSN:0008-543X
1097-0142
DOI:10.1002/1097-0142(197604)37:4<1814::AID-CNCR2820370428>3.0.CO;2-1