Half life of yeast messenger RNA

WE have determined the half life of Saccharomyces cerevisiae messenger RNA (mRNA) and its associated poly (A) sequences, from the kinetics of their labelling with 14 C-adenine. Corrections have been made for changes in the ATP pool specific activity during the labelling period. This method of determ...

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Bibliographic Details
Published in:Nature (London) 1976-03, Vol.260 (5546), p.70-72
Main Authors: PETERSEN, N. S, MCLAUGHLIN, C. S, NIERLICH, D. P
Format: Article
Language:English
Subjects:
Adenosine Triphosphate - metabolism
Cell Division
Guanosine Triphosphate - metabolism
Humanities and Social Sciences
letter
multidisciplinary
Poly A - metabolism
Polyribosomes - analysis
RNA, Messenger - isolation & purification
RNA, Messenger - metabolism
Saccharomyces cerevisiae - metabolism
Science
Science (multidisciplinary)
Temperature
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Summary:WE have determined the half life of Saccharomyces cerevisiae messenger RNA (mRNA) and its associated poly (A) sequences, from the kinetics of their labelling with 14 C-adenine. Corrections have been made for changes in the ATP pool specific activity during the labelling period. This method of determining the mRNA half life is preferred to observing the decay of mRNA in the presence of inhibitors because changes in the cell metabolism caused by an inhibitor may also change the mRNA half life 1 . One of our purposes in determining these half lives is for their use in the characterisation of temperature-sensitive mutants which are defective in macromolecular synthesis 2 . For this reason we have determined the half lives of mRNA and poly(A) in two different conditions, at 23 and 36 °C.
ISSN:0028-0836
1476-4687
DOI:10.1038/260070a0