Loading…

Isolation of the Monkey Brain: In vitro Preparation and Maintenance

Sustained viability of the primate brain, as a totally isolated organ preparation, was achieved by utilizing an extracorporeal (compatible donor) circulation. Five rhesus monkey brains, completely isolated neurogenically and vascularly, were perfused in vitro for 30 to 180 minutes. Retention of biol...

Full description

Saved in:
Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1963-09, Vol.141 (3585), p.1060-1061
Main Authors: White, Robert J., Albin, Maurice S., Verdura, Javier
Format: Article
Language:English
Subjects:
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Sustained viability of the primate brain, as a totally isolated organ preparation, was achieved by utilizing an extracorporeal (compatible donor) circulation. Five rhesus monkey brains, completely isolated neurogenically and vascularly, were perfused in vitro for 30 to 180 minutes. Retention of biological activity was evidenced by: (i) persistent electrocortical activity, and (ii) significant mean A-V$_{O_{2}}$ (5.8 volumes percent) and V-A$_{CO_{2}}$ (5.0 volumes percent) differences across the isolated brain.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.141.3585.1060