Isolation of the Monkey Brain: In vitro Preparation and Maintenance

Sustained viability of the primate brain, as a totally isolated organ preparation, was achieved by utilizing an extracorporeal (compatible donor) circulation. Five rhesus monkey brains, completely isolated neurogenically and vascularly, were perfused in vitro for 30 to 180 minutes. Retention of biol...

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Published in:Science (American Association for the Advancement of Science) 1963-09, Vol.141 (3585), p.1060-1061
Main Authors: White, Robert J., Albin, Maurice S., Verdura, Javier
Format: Article
Language:English
Subjects:
Animals
Blood
Brain
Brain - physiology
Cerebral Cortex
Cerebrovascular Circulation
Electrodes
Electrophysiology
Femoral artery
Haplorhini
Heart
Heart, Artificial
In Vitro Techniques
Indwelling catheters
Interferons
Metabolism
Monkeys
Old Medline
Perfusion
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cited_by cdi_FETCH-LOGICAL-c357t-e9481ee41b00fe6790620b8df9537a213c470374a2393e59489b777f06753e973
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creator White, Robert J.
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description Sustained viability of the primate brain, as a totally isolated organ preparation, was achieved by utilizing an extracorporeal (compatible donor) circulation. Five rhesus monkey brains, completely isolated neurogenically and vascularly, were perfused in vitro for 30 to 180 minutes. Retention of biological activity was evidenced by: (i) persistent electrocortical activity, and (ii) significant mean A-V$_{O_{2}}$ (5.8 volumes percent) and V-A$_{CO_{2}}$ (5.0 volumes percent) differences across the isolated brain.
doi_str_mv 10.1126/science.141.3585.1060
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source American Association for the Advancement of Science; JSTOR-E-Journals
subjects Animals
Blood
Brain
Brain - physiology
Cerebral Cortex
Cerebrovascular Circulation
Electrodes
Electrophysiology
Femoral artery
Haplorhini
Heart
Heart, Artificial
In Vitro Techniques
Indwelling catheters
Interferons
Metabolism
Monkeys
Old Medline
Perfusion
title Isolation of the Monkey Brain: In vitro Preparation and Maintenance
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