Cytochemical demonstration of extraperoxisomal catalase. I. Sheep liver

In sheep hepatocytes catalase activity was demonstrated both within peroxisomes and within the cytosol. In the cytosol the catalase reaction product is contiguous to the plasma membrane and surrounds the nuclei, rough endoplasmic reticulum, cisternae, mitochondria and Golgi apparatus. This is the fi...

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Bibliographic Details
Published in:The journal of histochemistry and cytochemistry 1976-06, Vol.24 (6), p.713-724
Main Author: Roels, F
Format: Article
Language:English
Subjects:
Animals
Catalase - metabolism
Cytosol - enzymology
Female
Histocytochemistry
Indicators and Reagents
Liver - enzymology
Liver - ultrastructure
Male
Mice
Microbodies - enzymology
Microbodies - ultrastructure
Microscopy, Electron
Peroxidases - metabolism
Sheep
Species Specificity
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Summary:In sheep hepatocytes catalase activity was demonstrated both within peroxisomes and within the cytosol. In the cytosol the catalase reaction product is contiguous to the plasma membrane and surrounds the nuclei, rough endoplasmic reticulum, cisternae, mitochondria and Golgi apparatus. This is the first cytochemical demonstration of guine extraperoxisomal catalase. No catalase reaction product was seen in the cytosol of nonparenchymal cells. To demonstrate catalase, both glutaraldehyde and formaldehyde fixation were used, followed by a diaminobenzidine technique modified from Novikoff and Goldfischer. Control reactions were performed to distinguish catalase reaction product from adsorption of oxidized diaminobenzidine and from precipitate due to oxidase-, peroxidase- or heat-stable peroxidatic activities. The results were evaluated in the light and electron microscopes.
ISSN:0022-1554
1551-5044
DOI:10.1177/24.6.950458