D-Glucosamine as inhibitor of early processes of transformation in Bacillus subtilis 168 trp2

Glucosamine added to a transformation medium (TM2) after a 30-min cultivation of cells exhibited the highest inhibitory effect on the transformation process in Bacillus subtilis 168 trp2. The recipient culture was least sensitive to glucosamine added after 50 min. Glucosamine had no inhibitory effec...

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Bibliographic Details
Published in:Folia microbiologica 1976, Vol.21 (2), p.100-106
Main Authors: Tichý, P, Rytír, V, Sobotková, E, Fuchsová, Z
Format: Article
Language:English
Subjects:
Bacillus subtilis - metabolism
DNA, Bacterial - metabolism
Glucosamine - pharmacology
Glucose - metabolism
Mutation
Stereoisomerism
Time Factors
Transformation, Genetic - drug effects
Tryptophan - biosynthesis
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Summary:Glucosamine added to a transformation medium (TM2) after a 30-min cultivation of cells exhibited the highest inhibitory effect on the transformation process in Bacillus subtilis 168 trp2. The recipient culture was least sensitive to glucosamine added after 50 min. Glucosamine had no inhibitory effect when added 10 min after the transformation DNA.
ISSN:0015-5632
DOI:10.1007/BF02876976