Relative activation of milk lipoprotein lipase by serum of cows fed varying amounts of fat

A routine laboratory assay to evaluate relative concentrations of lipoprotein lipase activator (apo C-II) in cow serum was developed. The assay was linear for at least 120 min after an initial, unexplained, lag ime of 13 to 15 min. Half-maximal activation was in the range of 1 to 2% serum in the ass...

Full description

Saved in:
Bibliographic Details
Published in:Journal of dairy science 1976-08, Vol.59 (8), p.1409-1413
Main Authors: Super, D.M, Palmquist, D.L, Schanbacher, F.L
Format: Article
Language:English
Subjects:
Animals
Apoproteins - blood
Cattle
Dietary Fats - administration & dosage
Enzyme Activation
Female
Lactation
Lipids - blood
Lipoprotein Lipase - metabolism
Milk - enzymology
Pregnancy
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A routine laboratory assay to evaluate relative concentrations of lipoprotein lipase activator (apo C-II) in cow serum was developed. The assay was linear for at least 120 min after an initial, unexplained, lag ime of 13 to 15 min. Half-maximal activation was in the range of 1 to 2% serum in the assay. Inhibition of activation was indicated at high amounts (10%) of serum. Activation from plasma was half that from serum, presumably caused by an increase in substrate Km in the presence of heparin. Use of glyceryl tri[9,10-3H] oleate yielded excessively high blanks; [2-3H] glyceryl triolein is suggested for routine assay. Relative amounts of activator were not different between dry and lactating cows fed "conventional" diets. Activator concentration increased linearly with increasing dietary fat and was related to concentration of total lipid in plasma. The assay may provide a useful adjunct in studies on lipoprotein metabolism.
ISSN:0022-0302
1525-3198
DOI:10.3168/jds.S0022-0302(76)84378-0