Assimilation of Amino Acid Analogues by a Virus-Induced Tumor

The uptake of labeled amino acids was studied in vitro with the use of the chorio-allantoic membrane (CAM) of embryonated eggs and Rous sarcoma virus-induced tumors of CAM. The two amino acids studied were α-aminoisobutyric acid (AIB) and l-aminocyclopentane-l-carboxylic acid (ACC). Both AIB and ACC...

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Bibliographic Details
Published in:JNCI : Journal of the National Cancer Institute 1964-03, Vol.32 (3), p.731-738
Main Authors: Love, William C., Levine, Alvin S., Ashmore, James
Format: Article
Language:English
Subjects:
Amino Acids
Aminoisobutyric Acids
Animals
Avian Sarcoma Viruses
Body Fluids
Carbon Isotopes
Chick Embryo
Extracellular Space
Extraembryonic Membranes
Histocytochemistry
Liver
Neoplasm Proteins
Old Medline
Oncogenic Viruses
Proteins - metabolism
Research Design
Rous sarcoma virus
Sarcoma, Avian
Tissue Culture Techniques
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Summary:The uptake of labeled amino acids was studied in vitro with the use of the chorio-allantoic membrane (CAM) of embryonated eggs and Rous sarcoma virus-induced tumors of CAM. The two amino acids studied were α-aminoisobutyric acid (AIB) and l-aminocyclopentane-l-carboxylic acid (ACC). Both AIB and ACC were concentrated in the intracellular fluid of tumor slices and CAM. Tumor tissue levels of these amino acids exceeded the level observed in the CAM. AIB and ACC concentration by Rous tumor and CAM was markedly inhibited by the presence of the natural amino acids, serine and valine. Both 2,4-dinitrophenol and anaerobiosis reduced AIB accumulation. Glucose suppressed the accumulation of AIB by tumor slices, but was without effect on the accumulation of the amino acid by CAM.
ISSN:0027-8874
1460-2105
DOI:10.1093/jnci/32.3.731