Permeability of a cell junction during intracellular injection of divalent cations

Divalent cations are microinjected into Chironomus salivary gland cells while the cell-to-cell passage of fluorescein (330 dalton) and electrical coupling are monitored. Injections of Ca and Mg that substantially depolarize the cells produce block or marked slowing fluorescein passage, accompanied b...

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Published in:The Journal of membrane biology 1976-12, Vol.28 (1), p.71-86
Main Authors: Délèze, J, Loewenstein, W R
Format: Article
Language:English
Subjects:
Animals
Calcium - pharmacology
Cell Membrane Permeability - drug effects
Diptera
Fluoresceins - metabolism
Intercellular Junctions - metabolism
Magnesium - pharmacology
Membrane Potentials - drug effects
Potassium - pharmacology
Salivary Glands - cytology
Strontium - pharmacology
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description Divalent cations are microinjected into Chironomus salivary gland cells while the cell-to-cell passage of fluorescein (330 dalton) and electrical coupling are monitored. Injections of Ca and Mg that substantially depolarize the cells produce block or marked slowing fluorescein passage, accompanied by electrical uncoupling. Injections of Ca, Mg or Sr that cause little depolarization, and presumably smaller elevation of divalent cation concentration in the cytoplasm, produce block or marked slowing of fluorescein passage with little or no detectable electrical uncoupling. This partial uncoupling may reflect total closure of a fraction of the channels in junctional membrane or partial closure of all channels.
doi_str_mv 10.1007/BF01869691
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subjects Animals
Calcium - pharmacology
Cell Membrane Permeability - drug effects
Diptera
Fluoresceins - metabolism
Intercellular Junctions - metabolism
Magnesium - pharmacology
Membrane Potentials - drug effects
Potassium - pharmacology
Salivary Glands - cytology
Strontium - pharmacology
title Permeability of a cell junction during intracellular injection of divalent cations
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