Progress of liquid chromatography-mass spectrometry in measurement of vitamin D metabolites and analogues

Clinical testing for vitamin D nutritional status has experienced tremendous growth in the past several years, driven by research results linking various diseases with low serum 25-hydroxyvitamin D [25(OH)D] levels. Meanwhile, interest in the pathophysiological mechanism elucidation and pharmaceutic...

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Bibliographic Details
Published in:Clinical biochemistry 2011, Vol.44 (1), p.66-76
Main Authors: El-Khoury, Joe M., Reineks, Edmunds Z., Wang, Sihe
Format: Article
Language:English
Subjects:
1,25-dihydroxyvitamin D
25-hydroxyvitamin D
Animals
Chromatography, Liquid - methods
Humans
LC-MS
Liquid chromatography
Mass spectrometry
Mass Spectrometry - methods
Molecular Structure
Vitamin D
Vitamin D - analogs & derivatives
Vitamin D - analysis
Vitamin D - metabolism
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Summary:Clinical testing for vitamin D nutritional status has experienced tremendous growth in the past several years, driven by research results linking various diseases with low serum 25-hydroxyvitamin D [25(OH)D] levels. Meanwhile, interest in the pathophysiological mechanism elucidation and pharmaceutical applications requires measurement of vitamin D metabolites and analogues. Liquid chromatography-mass spectrometry (LC-MS) has been increasingly utilized in these applications. In this work, our objective was to critically review the progress of LC-MS application in measuring vitamin D metabolites and analogues in biological fluids. The LC-MS methods included were selected from those searchable in PubMed up to January 2010. LC-MS has unique advantages in measuring various vitamin D metabolites and analogues due to its flexibility, sensitivity, and specificity. Despite some controversies over serum 25(OH)D tests, LC-MS will be used for standardizing serum 25(OH)D assays using reference materials available from the National Institute of Standards and Technology.
ISSN:0009-9120
1873-2933
DOI:10.1016/j.clinbiochem.2010.05.007