Effect of specimen storage conditions on newborn dried blood spots used to assess Toxoplasma gondii immunoglobulin M (IgM)

Newborn screening programs store—under varying conditions—residual dried blood spots (DBS). Residual DBS were used to investigate the contribution of congenital infection with Toxoplasma gondii to the etiology of hydrocephalus and as a key step, we assessed the effect of storage conditions on the st...

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Bibliographic Details
Published in:Clinica chimica acta 2011-02, Vol.412 (5-6), p.455-459
Main Authors: Mei, Joanne V., Li, LiXia, Rasmussen, Sonja A., Collier, Sarah, Frias, Jaime L., Honein, Margaret A., Shaw, Gary M., Lorey, Fred, Meyer, Robert, Chaing, Shu, Canfield, Mark A., Jones, Jeffrey, Hannon, W. Harry
Format: Article
Language:English
Subjects:
Animals
Blood Chemical Analysis - methods
Blood Specimen Collection - methods
Congenital hydrocephalus
Dried blood spots
Humans
Hydrocephalus - blood
Hydrocephalus - immunology
Hydrocephalus - parasitology
Immunoglobulin M - blood
Infant, Newborn
Newborn screening
Sensitivity and Specificity
Storage conditions
Toxoplasma - immunology
Toxoplasma - isolation & purification
Toxoplasma gondii IgM
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Summary:Newborn screening programs store—under varying conditions—residual dried blood spots (DBS). Residual DBS were used to investigate the contribution of congenital infection with Toxoplasma gondii to the etiology of hydrocephalus and as a key step, we assessed the effect of storage conditions on the stability of newborn screening biomarkers. Infants with hydrocephalus (410 cases) were identified using population-based birth defects surveillance systems in California, North Carolina, and Texas. Infants without birth defects (448 controls) were randomly selected from the same geographic areas and time periods. California stores DBS with controlled temperature, while North Carolina and Texas store DBS under ambient conditions. After removal of personal identifiers, DBS were tested for Toxo-specific immunoglobulin-M (Toxo-IgM). Because of poor elution of DBS stored in ambient conditions, additional biomarkers were tested on a specimen subset. Among 858 DBS tested, Toxo-IgM was found in 3 cases and no controls from California (N=515) and in no specimens from North Carolina or Texas (N=343). Among the 98 specimens tested for selected biomarkers, statistically significant differences were found for California vs. combined North Carolina and Texas DBS (thyroid stimulating hormone, phenylalanine, methionine, leucine and citrulline p
ISSN:0009-8981
1873-3492
DOI:10.1016/j.cca.2010.11.028