Diurnal variation and effect of insulin on circulating high molecular weight (HMW) adiponectin and NF-κB activity in human endothelial cells

Abstract Objective To study the diurnal variation and the effect of insulin on adiponectin multimers and nuclear factor-kappaB (NF-κB) activity in human endothelial cells. Methods and results We utilized a prolonged insulin–glucose infusion in six healthy human subjects. HMW and total adiponectin le...

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Bibliographic Details
Published in:Atherosclerosis 2011-01, Vol.214 (1), p.174-177
Main Authors: Tan, Bee K, Adya, Raghu, Lewandowski, Krzysztof C, O’Hare, J. Paul, Randeva, Harpal S
Format: Article
Language:English
Subjects:
Adipokine
Adiponectin
Adiponectin - blood
Adult
Atherosclerosis
Atherosclerosis (general aspects, experimental research)
Biological and medical sciences
Blood and lymphatic vessels
Blood Glucose - metabolism
Blood. Blood coagulation. Reticuloendothelial system
Cardiology. Vascular system
Cardiovascular
Circadian Rhythm
Diabetes
Diabetes Mellitus, Type 2 - blood
Endothelial Cells - metabolism
Female
Humans
Hyperinsulinism - metabolism
Insulin - blood
Insulin Resistance
Male
Medical sciences
Microcirculation
Models, Biological
Molecular Weight
NF-kappa B - metabolism
NF-κB
Obesity - blood
Pharmacology. Drug treatments
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Summary:Abstract Objective To study the diurnal variation and the effect of insulin on adiponectin multimers and nuclear factor-kappaB (NF-κB) activity in human endothelial cells. Methods and results We utilized a prolonged insulin–glucose infusion in six healthy human subjects. HMW and total adiponectin levels were higher in the morning and lower at night; NF-κB activities in serum treated human microvascular endothelial cells (HMEC-1) cells were lower in the morning and higher at night. Hyperinsulinemic induction significantly decreased HMW and total adiponectin levels but increased NF-κB activity in serum treated HMEC-1 cells ( P < 0.05, P < 0.01). There were no significant changes to MMW and LMW adiponectin levels ( P > 0.05). Conclusion Circadian rhythm of HMW adiponectin and NF-κB activity are altered by hyperinsulinemia providing novel insights adiponectin and NF-κB biology, which may be pertinent to insulin resistant states, e.g. obesity and type 2 diabetes mellitus.
ISSN:0021-9150
1879-1484
DOI:10.1016/j.atherosclerosis.2010.10.013